To determine whether the activation of EGFR, which is overexpressed or mutated in many types of cancer20, has an effect on PFKP expression, we used EGF to stimulate U251, LN229, and EGFR-overexpressed U87 (U87/EGFR) GBM cells, A431 human epidermoid carcinoma cells, and MDA-MB-231 human breast carcinoma cells. Here, EGF is linked to breast carcinoma.