Similar to the pattern of rPFKP protein expression levels, PFK activity, pyruvate kinase activity, lactate production (Fig. 6b), cell proliferation (Fig. 6c), brain tumor growth in mice (Fig. 6d), and Ki-67 expression levels in brain tumor tissues (Fig. 6e) were reduced by rPFKP S386A but enhanced by rPFKP K10R expression in U87/EGFRvIII cells. This evidence concerns the gene MKI67 and brain neoplasm.