An in vitro PFKP activity assay showed that WT rPFKP, rPFKP S386A, and rPFKP K10R had comparable activity (Supplementary Fig. 6a), suggesting that PFKP S386 phosphorylation and PFKP K10 ubiquitylation, which do not change the enzymatic activity of PFKP, regulate PFKP protein expression and thus glycolysis, cell proliferation, and brain tumor development. Here, PFKP is linked to brain neoplasm.