In our adaptation of this model, spheroids of syngenic C57Bl6 mouse CT2A (Seyfried et al, 1992; Oh et al, 2014) and GL261 (Szatmari et al, 2006) glioma cells modified to stably express blue fluorescent protein TagBFP (Subach et al, 2008) were engrafted in the endothelial‐specific Pdgfb‐iCre line (Claxton et al, 2008) and in Cdh5‐iCre (Sorensen et al, 2009) crossed with the ROSAmT/mG reporter mouse (Muzumdar et al, 2007). Here, CDH5 is linked to glioma.