Therefore, the purpose of this study was to investigate changes in blood glucose levels and insulin resistance during an 10 weeks treadmill exercise in a T2D animal model induced by a highfat diet, and to confirm the change of Beclin-1, which is involved in the autophagic initiation process, and LC3-II/I, p62, and LAMP-2, which are involved in the disintegration phase. The gene discussed is SQSTM1; the disease is type 2 diabetes mellitus.