One-way ANOVA of the insulin level showed a statistically significant difference between the ND-SED (6.55 ± 1.75), HF-SED (16.50 ± 3.58), and HF-TE (10.72 ± 2.75) groups [F (2, 21) = 25.453, p = .000]. The gene discussed is INS; the disease is hydrops fetalis.