It has been reported that probiotics of clostridium butyricum MIYAIRI 588—a butyrate-producing bacteria, decreased accumulation of lipid droplets in HFD-induced NAFLD models, improved insulin resistance [102], and reduced hepatic lipids and serum endotoxin levels in choline-deficient/l-amino acid-defined diet-induced NAFLD models [103], which may be associated with the stimulation of expression of adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) andserine/threonine kinas (AKT) proteins, and lipogenesis- or lipolysis-related proteins. This evidence concerns the gene AKT1 and metabolic dysfunction-associated steatotic liver disease.