Another study found that ectopic ATRX expression within telomerase-deficient, ALT-positive osteosarcoma epithelial (U-2 OS) cells led to DAXX-dependent reduction of several features of the ALT phenotype (58), signifying that ATRX loss is imperative for the maintenance of the ALT phenotype (58). The gene discussed is GPT; the disease is osteosarcoma.