Because in the past we developed the BIT system to generate ad hoc translocations without strain pre-engineering, just by exploiting the strong homologous recombination of yeast cells (11, 87), we decided to reproduce in S. cerevisiae a deeply characterized translocation in humans, responsible of AML, between NUP98 and TOP2B (13). This evidence concerns the gene NUP98 and acute myeloid leukemia.