As shown in Figures 7A,B, the expression of cytosolic cytochrome C and active cleaved caspase-9 were increased in GBM cells treated with DHA, and GBM cells pretreated with MnTMPyP significantly reduced these expression compared with DHA treatment alone, suggesting that DHA induced the mitochondrial pathway of apoptosis was mediated by ROS generation. This evidence concerns the gene CYCS and glioblastoma.