Further, the expression of autophagy-related genes, two MAP1-LC3 forms (LC3-I and LC3-II), p62/SQSTM1 and Beclin-1 were detected by Western blot, and results showed that increased conversion of LC3-I to LC3-II and increased Beclin-1 expression were observed in DHA treated GBM cells, whereas the reduced expression of p62/SQSTM1 was found in DHA treated GBM cells (Figures 4D–F). This evidence concerns the gene MAP1LC3A and glioblastoma.