Extrahepatic metastasis assessed in tail vein-injected nude mice demonstrated that NKCC1-overexpressing MHCC97L cells formed more metastatic tumor nodules in the lungs on average, whereas NKCC1-knockdown MHCC97H cells formed significantly fewer lung metastatic tumors, compared with control (Figure 4D and 4E). Here, SLC12A2 is linked to neoplasm.