Using Chi-Square test (with SPSS version 16.0 software), tumor differentiation and microvascular invasion were shown to have significant associations with NKCC1 expression (p<0.05, Table 1), while no significant association between NKCC1 expression and gender, age, glypican-3, keratin 19 or Ki-67. The gene discussed is KRT19; the disease is neoplasm.