Taken together, our study showed the status of amplification of PIK3CA and loss of PTEN in DLBCL on DNA level, observing that no significant association was found between genetic changes of PIK3CA and PTEN versus clinicopathological variables available to us, including overall prognosis, suggesting that amplification of PIK3CA and loss of PTEN was an event in the pathogenesis of DLBCL. This evidence concerns the gene PIK3CA and diffuse large B-cell lymphoma.