To further examine whether inhibition of FZD7 expression could suppress the ESCC cell mobility and tumor metastasis in mouse model, we knocked down the endogenous FZD7 expression in KYSE30 and KYSE410 cells (KYSE30/KYSE410-sh1/3) by lentiviral transduction. The gene discussed is FZD7; the disease is esophageal squamous cell carcinoma.