To verify the role of FZD7 in ESCC cells, we established NE1, NE3, EC109 and KYSE520 cells which show little endogenous FZD7 transcription with FZD7 stable expression (NE1/ NE3/EC109/KYSE520-FZD7) by lentiviral transduction. This evidence concerns the gene FZD7 and esophageal squamous cell carcinoma.