To explore the expression levels of TUG1 in CCA, qRT-PCR experiments were performed on 51 pairs of CCA tissues as well as diverse CCA cell lines including KMBC, HCCC-9810, HuCCT1, CCLP-1, Huh-28, QBC939 and RBE. This evidence concerns the gene PPFIBP2 and cholangiocarcinoma.