To explore the effect of VRK1 on the malignant ESCC phenotype, Ec9706 cells stably overexpressing full-length VRK1 DNA (OEVRK1-Ec9706) as well as Eca109 (shVRK1-Eca109) and Kyse150 (shVRK1 -Kyse150) cells transfected with VRK1-shRNA lentivirus were constructed. The gene discussed is VRK1; the disease is esophageal squamous cell carcinoma.