On multivariable analysis including pre-operative PSA level, pathologic tumor stage, Gleason score, lymph node status and margin status, PTEN loss by IHC remained significantly associated with decreased PSA recurrence-free survival, with a hazard ratio of 1.3 (95% CI:1.16-1.47), a hazard ratio roughly equivalent to that seen for positive lymph node status (Table 3). The gene discussed is PTEN; the disease is neoplasm.