Of the 169 cases analyzed, 55 (32.5%) showed positive Twist1 expression in both cancer cells and stromal fibroblasts, 15 (8.9%) showed negative Twist1 expression in both cancer cells and stromal fibroblasts, 2 (1.2%) showed positive Twist1 expression in cancer cells only, and 97 (57.4%) showed positive Twist1 expression in stromal fibroblasts only. The gene discussed is TWIST1; the disease is cancer.