We found the prostate cancer cells which transfected with PDK1 or PFKFB4 shRNA obtained slower closure of the scratched wound, and the relative cell migration of LNCaP (Figure 3A) and PC3 cells (Figure 3B) were decreased significantly compared with the cells infected negative vector control cells and blank cells. The gene discussed is PFKFB4; the disease is Familial prostate cancer.