Besides, a significant reduction in density of COX-1- positive mitochondria was also clearly appreciable in NH2htau-exposed neuronal cultures (one-way repeated-measures ANOVA followed by Bonferroni post-hoc test ***p <0, 0001, Supplementary Figure 4E-4F), just resembling the retrograde degeneration or “dying-back neuronal death” as detectable at prodromal AD stages [86, 87, 88]. Here, MT-CO1 is linked to Alzheimer disease.