In vivo, miR-21 was increased ∼2-fold (p < 0.05) after 1 week of rapamycin treatment (3mg/kg, MWF and harvested at 4h post final injection) in xenografts generated by subcutaneous inoculation of 621-101 cells (Figure 1C) or ERL4 (Tsc2-deficient ELT3 cells derived from an Eker rat uterine leiomyoma and stably expressing luciferase; Figure 1D) cells into immunocompromised mice. The gene discussed is TSC2; the disease is Uterine leiomyoma.