As can be seen, alteration of DNA methylation marks may contribute to production of proinflammatory mediators (ALOX5 induced LTB4 production), and have role in osteogenic transformation of VICs (H19 induced NOTCH1 downregulation and subsequent upregulation of RUNX2, BMP2, and OCN) while some of them demonstrate promising biomarker potentials for the prediction of AS status (analysis of dried blood spots of neonatal AS patients). The gene discussed is ALOX5; the disease is aortic valve stenosis.