We knocked down ABHD5 in PC3 cells with shRNAs and observed that knockdown of ABHD5 had negligible effects on the expression of E-Cadherin and Snail (Supplementary Figure 2A) and on cell invasion (Supplementary Figure 2B), which suggests that p53 is required for activation of EMT in PCa cells by ABHD5 loss. Here, TP53 is linked to posterior cortical atrophy.