Meanwhile, the secretion of IL-10 (predominant cytokine secreted by M2 macrophages) in the supernatant of tumor lysates also significantly decreased by 3.77 times for the H-MnO2-PEG/C&D-injected mice plus light irradiation (Fig. 6b), whereas the secretion of IL-12 (predominant cytokine secreted by M1 macrophages) in tumor showing significant upregulation (Fig. 6c), both indicating significant M2 to M1 polarization for TAM within tumors post chemo-PDT with H-MnO2-PEG/C&D. This evidence concerns the gene IL10 and neoplasm.