Obesity was induced by feeding the rats with 30% fat diet for 12 weeks, and in one group of animals, high fat diet was supplemented with the dry leaf powder of W. somnifera. Post regimen, the effect of co-supplementation of W. somnifera was studied on anxiety-like behavior induced by DIO and the animals were sacrificed to study the expression of marker proteins associated with reactive gliosis (Glial Fibrillary Acidic Protein (GFAP)) and inflammation (Iba1, PPARγ, TNFα, IL-1β, IL-6) in the hippocampus, piriform cortex (PC), and hypothalamus regions of the brain. This evidence concerns the gene TNF and Obesity.