Previous studies have shown that MLE inhibits preadipocyte differentiation and ameliorates HFD-induced obesity and dyslipidemia [17], and in STZ-induced non-obese diabetic rats, MLE could also inhibit hyperglycaemia and increase insulin secretion, resulting in an improved metabolic status when taken at a dose of 6000 mg/kg [18]. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.