As we know, apoptosis had a pivotal role in VAN-induced AKI.12, 35 Although recent study has demonstrated that inhibition of MBD2 suppressed H2O2 induced endothelial cells apoptosis through activating ERK1/2 to phosphorylate BCL-2,22 the role and mechanism of VAN-induced HK-2 cell apoptosis remains unclear. This evidence concerns the gene MAPK3 and acute kidney injury.