After the transduction of dE1-RGD/GFP/shMot, expressions of the major ECM components are significantly decreased in primary keloid spheroid, and knockdown of mortain can attenuate EGF/EGFR signaling pathway and TGF-β1/Smad pathway, which have a role in the fibrogenesis. The gene discussed is TGFB1; the disease is keloid.