In corresponding with this, SHCBP1 gene expression in the two tested SS cell lines, human osteosarcoma cell line (Saos2), human hepatocarcinoma cell line (HepG2) and human breast cancer cell line (MCF-7) were significant upregulated (Fig. 1b), which is consistent with previous studies [19, 20]. Here, SHCBP1 is linked to osteosarcoma.