To quantitatively analyze the level of HCMV replication in HFF cells stably transduced with pLKO.1-STING shRNA, cells were infected with the HCMV-Towne at low (0.1 pfu/cell) or high (1 pfu/cell) multiplicity of infection (MOI), and the relative amount of viral DNA was measured by qPCR using primers specific for UL123 at 5 days after infection. The gene discussed is STING1; the disease is infection.