Since CRISPR/Cas9 KD of IFI16 has no effect on HCMV-induced TBK1 activation, IFI16 is not required for STING-TBK1-IRF3 activation in human fibroblasts upon HCMV infection (Diner et al., 2016). The gene discussed is IRF3; the disease is cytomegalovirus infection.