To investigate the effect of genetic reduction of GAD67 on Alzheimer's disease (AD), we crossed the 5xFAD transgenic mice with GAD67-GFP+/− heterozygotes to generate four different genotypes of littermates: wild type (AD-GG-), GAD67-GFP+/− (AD-GG+), 5xFAD (AD+GG-) and 5xFAD/GAD67-GFP+/− (AD+GG+, bigenic mice). Here, GAD1 is linked to early-onset autosomal dominant Alzheimer disease.