Accompanying the changes of Aβ plaques, we also observed a significant reduction of neuronal density surrounding the Aβ plaques in the FCX of 5xFAD mice (10–11 months old), but such neuronal loss was partially rescued in bigenic mice (AD+GG+) (Additional file 1: Figure S2), suggesting that Gad67 haploinsufficiency not only reduces amyloid plaques but also preserves surrounding neurons in the 5xFAD mouse model. This evidence concerns the gene GAD1 and Alzheimer disease.