In order to analyze the binding of KLF4 in IGF2-DMR0 as a function of the methylation status, we analyzed two prostate cancer cell lines LNCaP and DU145, which exhibited an opposite methylation status in IGF2-DMR0 (LNCaP: average 17% methylation; DU145: 85%) (Fig. 6a) and expressed the transcription factor KLF4 at mRNA and protein levels (Fig. 6c1 and 2, respectively). Here, IGF2 is linked to Familial prostate cancer.