Indeed, since AGO2 is not required for ERβ binding to BC cell genome, as silencing of this factor did not prevent in a significant way receptor binding to chromatin (data not shown), it is possible to assume that the ERβ–AGO2 complex detected in Ct-ERβ cell nuclear extracts by interaction proteomics is present also on the chromatin, where it could be conveyed by the receptor to specific sites, which are mainly distributed around TSSs (Fig. 7b), within promoters and 5′ UTRs (FC > 2 and q-value ≤ 0.05: Fig. 7c). Here, ESR2 is linked to breast cancer.