Considering that ERβ–AGO2 co-occupancy occurs at 858 sites in chromatin, and the fact that the chromatin-bound nuclear receptor can exert transcriptional effects also through long-range chromatin looping, the results reported above strongly suggest a functional role of the cooperation between ERβ and AGO2 on direct regulation of gene transcription and co-transcriptional RNA splicing in BC cells. Here, ESR2 is linked to breast cancer.