In consistent with the results from in vitro study, we found that the levels of Wnt1, phosphorylated GSK3β and active β‐catenin, Slug and Snail were significantly increased in poorly cohesive carcinoma tissues by Western blot (Fig. 8A) and immunohistochemical staining (Fig. 8B) analyses compare with adjacent non‐tumour tissues. This evidence concerns the gene GSK3B and carcinoma.