Pathway analysis of the contents of control EVs using IPA identified the following pathways; clatherin-mediated endocytosis signalling, systemic lupus erythematosus signalling, LXR/RXR (liver X receptor/retinoid X receptor) activation, atherosclerosis signalling and FXR/RXR (farnesoid X receptor/retinoid X receptor) activation (Figure 5b). The gene discussed is NR1H4; the disease is atherosclerosis.