AKT1 and breast carcinoma: In human breast cancer cell lines, rapamycin and A-443654 combination induced G2-M arrest and apoptosis.41 Use of perifosine and temsirolimus to treat mice with gliomas resulted in decreased Akt and mTOR signaling, decreased proliferation and increased cell death.42 All listed preclinical studies taken together demonstrate that inhibition of multiple components of Akt/mTOR pathway is superior to inhibition of a single target.