In order to compare the ability of mIDH1 inhibitors to induce differentiation of AML cells, we implemented a cell differentiation assay using human THP1 leukemia cells engineered to express either wild type IDH1 (THP1-WT) or mIDH1-R132H (THP1-R132H) under a doxycycline-inducible promoter35,38. Here, GLI2 is linked to acute myeloid leukemia.