To investigate why the untreated contralateral tumor in the aFP group did not shrink even though epitope specific CD8+ T lymphocytes were developed in the aFP-treated tumor, we measured the number of CD3+, CD4+, CD8+, epitope specific CD8+ T cells, and Foxp3+ Tregs inside the untreated contralateral tumor using flow cytometry 12 days after aFP treatment. The gene discussed is CD4; the disease is neoplasm.