Even though the PD-1/PD-L1 axis was blocked by anti-PD-1 inhibitor and the high frequency of the epitope specific CD8+ T cells was observed, complete remission of the untreated contralateral tumor was observed in fewer cases than the aFP-treated tumors in the aFP + anti-PD-1 group in the two tumor mouse model. This evidence concerns the gene CD8A and neoplasm.