Although all samples expressed CD33, only three samples (Leu 14, BMI 1690, and BMI 1808) expressed CD33 within the CD45lo compartment, suggesting that Leu 14, BMI 1690, and BMI 1808 are positive for AML leukemic blasts (Additional file 1: Figure S1a). The gene discussed is CD33; the disease is acute myeloid leukemia.