We further validated that blocking PRC2 using the inhibitor DZNep increased the mRNA expression of ZFHX3. These data suggest that PRC2-silencing plays an important role in tumor cell differentiation that can be measured by ZFHX3. Mutation of ZFHX3 has been reported for HR-NB and autism [37, 57], however, its functional impacts remain unclear. Here, ZFHX3 is linked to autism.