To define the roles of NFAT5 and SBF2-AS1 in tumor growth ability in vivo, we established mouse xenograft models with NFAT5 knockdown, SBF2-AS1 knockdown and NFAT5 and SBF2-AS1 dual-knockdown GBM cells by subcutaneous injection. This evidence concerns the gene SBF2 and glioblastoma.