MAP1LC3A and colorectal carcinoma: To determine that BA could also induce autophagy flux in CRC cells, the colocalization of LC3 and LAMP1 was firstly analyzed by indirect immunofluorescence assay, which is often used as a marker for autophagy flux.7 The results showed that the colocalization of LC3 and LAMP1 was increased in BA-treated CRC cells compared with the control group (Figure 3a).