Half-life of mature-Cathepsin S was 16–18 h, suggesting that mature-Cathepsin S is more stable than pro-Cathepsin S. Nissler et al. found that E-64, a cysteine protease inhibitor, can inhibit cleavage and degradation of Cathepsin L. These publications provide the evidences that Cathepsins degradation in lysosome occurs naturally.42, 43 In our results, hispolon inhibited both pro- and mature-Cathepsin S in cervical cancer cells by autophagy (Figure 5b and Supplementary Figure S2). Here, CTSB is linked to cervical cancer.