These cell lines, like PC3 are TRAIL-sensitive.25, 26, 27 HeLa and LNCaP (prostate cancer) cells have limited sensitivity to TRAIL8, 28 but their sensitivity can be enhanced by cycloheximide (CHX).29, 30 As expected, treatment of these cells with TRAIL alone produced very little PARP cleavage (Supplementary Figures S3A, S3D and S4E–F) when compared with TRAIL-sensitive cells (Figure 1d, and Supplementary Figures S2C–D). This evidence concerns the gene TNFSF10 and prostate cancer.