We quantified the majority of key proteins associated with classical complement pathway, such as C1q, C1s, C1r, C4a, C4b, C3, C5, C6, C7, C8a, C8b, C8g and C9, as up-regulated in glaucoma condition for both vitreous and retinal tissues when compared to the controls. The gene discussed is C4B; the disease is glaucoma.