In order to figure out the effects of the suppressed autophagy of ASMs on asthma development or airway hypersensitivity, we generated Adeno-associated viruses (AAVs) carrying miR-384 (AAV-miR-384) or null (AAV-CTL) under control of an α-SMA promoter, to specifically express the transgene in ASM cells (Figure 3A). Here, ACTA1 is linked to asthma.