In the current study, we evaluated the outperformance of digital PCR in the identification of low frequency EGFR L858R, T790M and exon 19 deletion mutations in tumor tissue samples from advanced lung adenocarcinoma patients through comparison with those detected using Ion AmpliSeq Cancer Hotspot Panel V2 (Thermo Fisher Scientific, Waltham, MA) and ARMS PCR. This evidence concerns the gene EGFR and lung adenocarcinoma.