IHC analysis of tumour sections confirmed the elevation of the proliferation marker Ki-67 and stromal Gli-1 expression, as well as the increase in α-SMA staining and resultant expression of the lymphatic marker LYVE1, in the tumours from mice co-injected with Caov3 SHH-OE cells and CAFs compared with that in the tumours from mice in co-injected with Caov3 NC cells and CAFs (Figure 6D). Here, GLI1 is linked to neoplasm.