Four shRNA clones generated a significant down-regulation of MYSM1 on the RNA and protein level in the analyzed mixed populations of sorted GFP-positive A375 MYSM1-knockdown cells (termed shRNA clones d, e, f and h) as compared with the scrambled control RNA expressing and parental A375 melanoma cells (Figure 4A, Supplementary Figure 4) and were used for further analyses. This evidence concerns the gene MYSM1 and melanoma.