In order to investigate if MYSM1 – similar to transcriptional regulators like PAX3 or ETS1 – may be critical for melanoma cell survival or proliferation, we subsequently subjected two melanoma cell lines, A375 and SK-MEL-28, to lentiviral transduction with different MYSM1 shRNA clones designed to stably silence MYSM1 expression. This evidence concerns the gene PAX3 and melanoma.