GLI1 and lung adenocarcinoma: Interestingly, in the fourth track, the significant presence of RNA Pol II on GLI-1 gene promoter sequences suggested that the GLI-1 gene is transcriptionally active, given the presence of the MEOX2 transcription factor, as well as epigenetically altered, demonstrated by a histone activation profile involving H3K4me3 and H3K27Ac and active enzyme RNA Pol II, which were previously identified in lung adenocarcinoma cells A549 obtained from the ENCODE project database analyzed here (Figure 3B).