In contrast, decreased enrichment of the repressive histones H3K27me3 and H3K9me3 in both lung adenocarcinoma patients and the lung adenocarcinoma cell lines A427 and A549 was also detected on GLI-1 gene promoter (data not shown); instead an increased active histone profiling may also be confirmed for GLI-1 gene and promoter sequences in A549 lung cancer cells based on the bioinformatics analyses obtained from the ENCODE project database (Figure 3B). This evidence concerns the gene GLI1 and lung adenocarcinoma.