GLI1 and lung cancer: In contrast, decreased enrichment of the repressive histones H3K27me3 and H3K9me3 in both lung adenocarcinoma patients and the lung adenocarcinoma cell lines A427 and A549 was also detected on GLI-1 gene promoter (data not shown); instead an increased active histone profiling may also be confirmed for GLI-1 gene and promoter sequences in A549 lung cancer cells based on the bioinformatics analyses obtained from the ENCODE project database (Figure 3B).