AKT1 and liver cancer: To examine the effects of AR regulated hepatic Akt/mTor signaling, lactate formation, inflammatory response gene expression and liver cancer development in vivo, HCC was induced in 2-wk old WT/FVB, TG2/FVB, TG1/FVB, WT/B6 and KO/B6 [47, 48] male mice by a single injection of DEN at the dosage of 25 mg/kg body weight.