Further studies have shown that PFKFB3 mRNA was elevated in gastric cancer tissue compared to normal gastric tissue, as well as in gastric cancer cell lines (MKN45, NUGC3) [35], and in head and neck squamous cell carcinoma (HNSCC) compared with adjacent mucosal tissue [36]. This evidence concerns the gene PFKFB3 and head and neck squamous cell carcinoma.