Taken together, this study demonstrated the functional and mechanistic relationship of the miR-135b-5p-SFRP4-Wnt/beta-catenin axis, and due to its effect on the proliferation, migration and apoptosis of pancreatic cancer miR-135b-5p and SFRP4 cells, components of this axis may be promising biomarkers for the prediction of clinical behaviors and outcomes in patients with PDAC. Here, SFRP4 is linked to pancreatic neoplasm.