To elucidate the mechanism of MT1E down-regulation in PCa, two MT1E fragments (MT1E-1 and MT1E-2; Supplementary Figure 2) were analyzed by means of methylation-specific PCR (MSP) in most of the cases from PCa1 and PCa2 cohorts (114 PCa and 24 NPT, overall). Here, MT1E is linked to posterior cortical atrophy.